nebnext ultra ii directional rna seq kit  (New England Biolabs)

Journal: Bioactive Materials

Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury

doi: 10.1016/j.bioactmat.2026.02.056

Figure Lengend Snippet: Mechanism-specific prediction of TBI treatment by EG-gel. (A) Principal component analysis (PCA) of Sham, TBI, and EG-gel groups in transcriptomic space. (B) Volcano plots of differentially expressed genes for Sham vs TBI and TBI vs EG-gel comparisons. Red and blue points indicate significantly up and downregulated genes. (C) Venn diagrams of mRNA expression among the three groups. (D) Heat map of differentially expressed genes among the three groups. (E) KEGG pathway analysis for differentially expressed genes. (F) The top 20 of KEGG terms enrichment of RNA sequences. (G) Bar chart of the top 20 enriched GO terms of RNA sequences. RNA-seq was performed on peri-injury cortical tissue (n = 6 per group), differential expression was called using fold change ≥1.5, p-value <0.05, q-value <0.05, and group mean FPKM ≥0.5.

Article Snippet: For library construction, 1–2 μg of total RNA per sample was used. mRNA was enriched using oligo (dT) selection, and libraries were prepared using the KAPA Stranded RNA-Seq Library Prep Kit (Roche).

Techniques: Expressing, RNA Sequencing, Quantitative Proteomics

Journal: Planta

Article Title: A chromosome-scale reference genome and integrative transcriptome provide insight into tissue- and stress-specific responses in tetraploid sainfoin (Onobrychis viciifolia)

doi: 10.1007/s00425-026-05021-y

Figure Lengend Snippet: Transcriptome sampling and tissue-specific gene expression patterns. a Principal component analysis (PCA) of RNA-Seq libraries from 9 tissues. Samples cluster by tissue type, with clear separation between root (RT, RC), flower (FB, OF), stem, leaves (DL, EL, 1st L, and 3rd L). b Flavonoid to condensed tannin biosynthetic pathway with enriched enzymes highlighted. Enzymes encoded by genes identified as tissue-enriched in sainfoin are highlighted in green (young leaves), blue (stem), and grey (roots). PAL phenylalanine ammonia-lyase, C4H cinnamate-4-hydroxylase, 4CL 4-coumarate:CoA ligase, CHS chalcone synthase, CHI chalcone isomerase, F3H flavanone 3-hydroxylase, F3′5’H flavonoid 3’, 5’-hydroxylase, DFR dihydroflavonol reductase, ANS anthocyanidin synthase, ANR anthocyanidin reductase, LAR leucoanthocyanidin reductase, OMT O-methyltransferase, UGT UDP-glycosyltransferase

Article Snippet: In the case of RNA-Seq, stranded libraries were prepared using Novogene’s RNA-Seq library construction workflow and sequencing (150 bp paired-end reads) was carried out on an Illumina NovaSeq 6000 platform.

Techniques: Sampling, Gene Expression, RNA Sequencing

Journal: Planta

Article Title: A chromosome-scale reference genome and integrative transcriptome provide insight into tissue- and stress-specific responses in tetraploid sainfoin (Onobrychis viciifolia)

doi: 10.1007/s00425-026-05021-y

Figure Lengend Snippet: Transcriptomic responses to drought and waterlogging stress in sainfoin. a Principal component analysis (PCA) of RNA-Seq samples from leaves derived from control (C), drought (D), and waterlogged (W) plants. b Volcano plots showing differentially expressed genes (DEGs) between drought vs. control (left) and waterlogging vs. control (right). Genes with log₂ fold-change > 1 and adjusted P -value < 0.05 are highlighted in red. c Venn diagrams showing overlap of up-regulated and down-regulated DEGs between drought and waterlogging treatments. Substantial overlap suggests a shared transcriptional response to both types of water stress

Article Snippet: In the case of RNA-Seq, stranded libraries were prepared using Novogene’s RNA-Seq library construction workflow and sequencing (150 bp paired-end reads) was carried out on an Illumina NovaSeq 6000 platform.

Techniques: RNA Sequencing, Derivative Assay, Control